Lab-on-a-chip Dielectrophoretic Manipulation of Beta-2 microglobulin for Toxin Removal in An Artificial Kidney


Ahmad Kayani, Aminuddin and Zoolfakar, Ahmad Sabirin and Hamzah, Azrul Azlan and Yeop Majlis, Burhanuddin and Buyong, Muhamad Ramdzan and Abd Samad, Muhammad Izzuddin (2019) Lab-on-a-chip Dielectrophoretic Manipulation of Beta-2 microglobulin for Toxin Removal in An Artificial Kidney. Micro and Nanosystems, 11 (1). pp. 40-46. ISSN 1876-4029

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Background: This paper presents a fundamental study of protein manipulation under the influence of dielectrophoretic (DEP) force for a lab-on-a-chip platform. Objective: Protein manipulation is dependent on the polarisation factor of protein when exposed to an electric field. Therefore the objective of this work is a microfluidic device and measurement system are used to characterise the human beta-2 microglobulin (β2M) protein via lateral attractive forces and vertical repulsive forces by means of DEP responses. Method: The manipulation of the β2M protein was conducted using a microfluidic platform with a tapered DEP microelectrode and the protein concentration was quantified based on a biochemical interaction using an Enzyme-Linked Immunosolvent Assay (ELISA). The protein distribution has been analysed based on the β2M concentration for each microfluidic outlet. Results: At 300 kHz, the protein experienced a negative DEP (nDEP) with of 83.3% protein distribution on the middle microchannel. In contrast, the protein experienced a positive DEP (pDEP) at 1.2 MHz with of 78.7% of protein on the left and right sides of the microchannel. Conclusion: This is concept proved that the tapered DEP microelectrode is capable of manipulating a β2M via particle polarisation, hence making it suitable to be utilised for purifying proteins in biomedical application.

Item Type: Article
Uncontrolled Keywords: Dielectrophoresis, Beta-2-microglobulin, Lab on chip, artificial kidney, hollow fibre membrane, end-stage renal disease.
Subjects: Q Science > QC Physics > QC501-766 Electricity and magnetism > QC501-(721) Electricity
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Suzilawati Abu Samah
Date Deposited: 25 Feb 2022 00:40
Last Modified: 25 Feb 2022 00:40


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