Citation

Ahmad Kayani, Aminuddin and Zoolfakar, Ahmad Sabirin and Hamzah, Azrul Azlan and Yeop Majlis, Burhanuddin and Buyong, Muhamad Ramdzan and Abd Samad, Muhammad Izzuddin (2019) Lab-on-a-chip Dielectrophoretic Manipulation of Beta-2 microglobulin for Toxin Removal in An Artificial Kidney. Micro and Nanosystems, 11 (1). pp. 40-46. ISSN 1876-4029

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Abstract

Background: This paper presents a fundamental study of protein manipulation under the influence of dielectrophoretic (DEP) force for a lab-on-a-chip platform. Objective: Protein manipulation is dependent on the polarisation factor of protein when exposed to an electric field. Therefore the objective of this work is a microfluidic device and measurement system are used to characterise the human beta-2 microglobulin (β2M) protein via lateral attractive forces and vertical repulsive forces by means of DEP responses. Method: The manipulation of the β2M protein was conducted using a microfluidic platform with a tapered DEP microelectrode and the protein concentration was quantified based on a biochemical interaction using an Enzyme-Linked Immunosolvent Assay (ELISA). The protein distribution has been analysed based on the β2M concentration for each microfluidic outlet. Results: At 300 kHz, the protein experienced a negative DEP (nDEP) with of 83.3% protein distribution on the middle microchannel. In contrast, the protein experienced a positive DEP (pDEP) at 1.2 MHz with of 78.7% of protein on the left and right sides of the microchannel. Conclusion: This is concept proved that the tapered DEP microelectrode is capable of manipulating a β2M via particle polarisation, hence making it suitable to be utilised for purifying proteins in biomedical application.

Item Type:	Article
Uncontrolled Keywords:	Dielectrophoresis, Beta-2-microglobulin, Lab on chip, artificial kidney, hollow fibre membrane, end-stage renal disease.
Subjects:	Q Science > QC Physics > QC501-766 Electricity and magnetism > QC501-(721) Electricity
Divisions:	Faculty of Engineering and Technology (FET)
Depositing User:	Ms Suzilawati Abu Samah
Date Deposited:	25 Feb 2022 00:40
Last Modified:	25 Feb 2022 00:40
URII:	http://shdl.mmu.edu.my/id/eprint/9191

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