Citation

Tan, Joon Liang and Yap, Sook Fan and Ngeow, Yun Fong and Ng, Hien Fuh and Zin, Thaw (2018) A mutation in anti-sigma factor MAB_3542c may be responsible for tigecycline resistance in Mycobacterium abscessus. Journal of Medical Microbiology, 67 (12). pp. 1676-1681. ISSN 0022-2615

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Abstract

In this study, we characterized 7C, a spontaneous mutant selected from tigecycline-susceptible Mycobacterium abscessus ATCC 19977. Whole-genome sequencing (WGS) was used to identify possible resistance determinants in this mutant. Compared to the wild-type, 7C demonstrated resistance to tigecycline as well as cross-resistance to imipenem, and had a slightly retarded growth rate. WGS and subsequent biological verifications showed that these phenotypes were caused by a point mutation in MAB_3542c, which encodes an RshA-like protein. In Mycobacterium tuberculosis, RshA is an anti-sigma factor that negatively regulates the heat/oxidative stress response mechanisms. The MAB_3542c mutation may represent a novel determinant of tigecycline resistance. We hypothesize that this mutation may dysregulate the stress-response pathways which have been shown to be linked to antibiotic resistance in previous studies.

Item Type:	Article
Uncontrolled Keywords:	Genomes, MAB_3542c, anti-sigma factor, RshA, tigecycline resistance, Mycobacterium abscessus
Subjects:	Q Science > QH Natural history
Divisions:	Faculty of Information Science and Technology (FIST)
Depositing User:	Ms Suzilawati Abu Samah
Date Deposited:	30 Nov 2020 08:44
Last Modified:	30 Nov 2020 08:44
URII:	http://shdl.mmu.edu.my/id/eprint/7669

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