TAU Protein cell characterisation towards establishing microfluidic platforms

Citation

Da En, Zuriel Shee (2022) TAU Protein cell characterisation towards establishing microfluidic platforms. Masters thesis, Multimedia University.

Full text not available from this repository.
Official URL: http://erep.mmu.edu.my/

Abstract

A dielectrophoresis (DEP) study on Tau-441 protein has been performed for lab-on-a-chip bioparticle manipulation investigation. In this work, a study of bioparticles manipulation which targeted on recombinant human Tau-441 protein under the gold rectangular shape microelectrode platform has been conducted with employing dielectrophoresis (DEP) technique to investigate and characterise this Tau-441 protein which is a protein in the brain neurons that is associated with Alzheimer’s disease (AD). Series of characterisation of recombinant human Tau-441 protein has been conducted. The morphology and structure of the Tau-441 protein have been examined with optical microscopy and fluorescence microscopy analysis with an average length of 206.62µm. Through Fourier-Transform Infrared Spectroscopy, Tau-441 protein was misfolded into pair helical filaments (PHF) and neurofibrillary tangles (NFTs) forms and 84.28% of its secondary structure is made up of β-Sheet structure. An electrochemical impedance spectroscopy was performed to identify the Tau-441 dielectric properties, the average of conductivity of Tau-441 protein calculated is 1.046 S/m. A parametric study on the rectangular shape microelectrode was conducted by using COMSOL® simulation analysis, the optimum microelectrode design parameters studied is a 130µm × 130µm rectangular shape gold microeletrode and was fabricated by using photolithography technique. A DEP test on model yeast cells was conducted and manipulated with a positive DEP in 60 second at 5 peak to peak voltage (Vpp) with 5MHz frequency. Next, two DEP configurations experiments were conducted on Tau-441 protein which were suspended in deionised water (DIW) and artificial cerebrospinal fluid (CSF) which is the brain fluid. Both experiments shown a negative DEP response at 5V with 10MHz. Lastly, DEP separation experiment was conducted on the combination of model yeast cells representing AD infected brain cells and Tau-441 protein which is the AD associated protein in the CSF medium. A successful separation of model yeast cells and Tau-441 protein mixture was achieved at 5Vpp with 10MHz. Overall, this research has successfully trapped and separate Tau-441 protein aggregates with model yeast cells by using dielectrophoresis technique with a separation manipulation efficiency up to 90%. The knowledge from these investigations has brought significant insight for research related to establishing dielectrophoresis microfluidic brain-on-chip platforms relevant to Alzheimer’s disease.

Item Type: Thesis (Masters)
Additional Information: Call No.: QC585.7.D5 Z87 2022
Uncontrolled Keywords: Dielectrophoresis
Subjects: Q Science > QC Physics > QC501-766 Electricity and magnetism > QC501-(721) Electricity
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Nurul Iqtiani Ahmad
Date Deposited: 18 Jul 2023 01:48
Last Modified: 18 Jul 2023 01:48
URII: http://shdl.mmu.edu.my/id/eprint/11537

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