Citation

Santhis Kumar, D. and Bhavitha, K. and Harani, A. and Meenakshi, V. and Rajendran, Parvathy and Lee, It Ee and Al Awadh, Mohammed and Lu, Yuheng and Prasad, N.E Chandra (2025) Green analytical comparison and central composite design optimization for simultaneous estimation of pain management drugs using RP-liquid chromatography. Microchemical Journal, 208. p. 112309. ISSN 0026265X

Text Green analytical comparison and central composite design optimization for simultaneous estimation of pain management drugs using RP-liquid chromatography.pdf - Published Version Restricted to Repository staff only Download (7MB)

Abstract

The Central Composite Design method was utilized to validate a precise RP-HPLC method for concurrently determining the quantities of Paracetamol (PC), Diclofenac Sodium (DS), and Eperisone Hydrochloride (EH) in tablet compositions. By employing Design of Experiment (DOE), the experimental parameters were fine-tuned, resulting in an optimized eluent consisting of methanol: water (90:10) with 0.1 % orthophosphoic acid at a eluent velocity of 1 mL/min. The method exhibited exceptional purities: PC (100.83 % ± 0.85), DS (102.01 % ± 0.90), and EH (100.49 % ± 1.29). Regression equations were formulated for PC, DS, and EH as follows: y = 479762x + 151907, y = 2182788x + 2409442, and y = 777144x − 1146334, respectively. The analytical method underwent comprehensive validation, including tests for: Accuracy, Precision, Linearity and Robustness. To assess the method’s environmental impact, several Green Analytical Chemistry (GAC) tools were employed. These tools provided a multifaceted evaluation of the method’s sustainability and eco-friendliness

Item Type:	Article
Uncontrolled Keywords:	Liquid chromatography, drugs used in pain management
Subjects:	Q Science > QD Chemistry > QD241-441 Organic chemistry T Technology > TX Home economics > TX341-641 Nutrition. Foods and food supply
Divisions:	Faculty of Engineering (FOE)
Depositing User:	Ms Rosnani Abd Wahab
Date Deposited:	18 Feb 2025 03:21
Last Modified:	18 Feb 2025 05:45
URII:	http://shdl.mmu.edu.my/id/eprint/13472

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