Dielectrophoretic deformation of breast cancer cells for lab on a chip applications


Chan, Jun Yuan and Kok, Chee Kuang and Ahmad Kayani, Aminuddin and Buyong, Muhamad Ramdzan and Hoe, Susan Ling Ling and Marzuki, Marini and Khoo, Alan Soo Beng and Sriram, Sharath and Ostrikov, Kostya (Ken) (2019) Dielectrophoretic deformation of breast cancer cells for lab on a chip applications. Electrophoresis. ISSN 0173-0835

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This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA-MB-231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA-MB-231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA-MB-231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field.

Item Type: Article
Uncontrolled Keywords: Blebbing, cancer, cell shrinkage, deformation, dielectrophoresis , microfluidics
Subjects: Q Science > QC Physics > QC501-766 Electricity and magnetism
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Suzilawati Abu Samah
Date Deposited: 08 Mar 2022 01:30
Last Modified: 08 Mar 2022 01:30
URII: http://shdl.mmu.edu.my/id/eprint/9236


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