Dielectrophoresis of Amyloid-Beta Proteins as a Microfluidic Template for Alzheimer’s Research


Ahmad Kayani, Aminuddin and Al-Ahdal, Salman Ali and Jun, Yuan Chan and Mhd Noor, Ervina Efzan and Md Ali, Mohd Anuar and Buyong, Muhamad Ramdzan and Ali, Talal and Adnan, Norah and Majlis, Burhanuddin Yeop and Sriram, Sharath (2019) Dielectrophoresis of Amyloid-Beta Proteins as a Microfluidic Template for Alzheimer’s Research. International Journal of Molecular Sciences, 20 (14). ISSN 1661-6596

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We employed dielectrophoresis to a yeast cell suspension containing amyloid-beta proteins (Aβ) in a microfluidic environment. The Aβ was separated from the cells and characterized using the gradual dissolution of Aβ as a function of the applied dielectrophoretic parameters. We established the gradual dissolution of Aβ under specific dielectrophoretic parameters. Further, Aβ in the fibril form at the tip of the electrode dissolved at high frequency. This was perhaps due to the conductivity of the suspending medium changing according to the frequency, which resulted in a higher temperature at the tips of the electrodes, and consequently in the breakdown of the hydrogen bonds. However, those shaped as spheroidal monomers experienced a delay in the Aβ fibril transformation process. Yeast cells exposed to relatively low temperatures at the base of the electrode did not experience a positive or negative change in viability. The DEP microfluidic platform incorporating the integrated microtip electrode array was able to selectively manipulate the yeast cells and dissolve the Aβ to a controlled extent. We demonstrate suitable dielectrophoretic parameters to induce such manipulation, which is highly relevant for Aβ-related colloidal microfluidic research and could be applied to Alzheimer’s research in the future.

Item Type: Article
Uncontrolled Keywords: Dielectrophoresis, microfabrication bioelectric, microfluidics, microanalysis, lab-on-a-chip
Subjects: Q Science > QC Physics > QC501-766 Electricity and magnetism
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Suzilawati Abu Samah
Date Deposited: 24 Feb 2022 03:47
Last Modified: 24 Feb 2022 03:47
URII: http://shdl.mmu.edu.my/id/eprint/9182


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