Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies


Ahmad Kayani, Aminuddin and Yeo, Leslie Y and Ahmad, Muhammad Zamharir and Majlis, Burhanuddin Yeop and Md Ali, Mohd Anuar and Chrimes, Adam F and Ostrikov, Kostya (Ken) (2018) Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies. Biomedical Microdevices, 20 (95). ISSN 1387-2176

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Cell contact formation, which is the process by which cells are brought into close proximity is an important biotechnological process in cell and molecular biology. Such manipulation is achieved by various means, among which dielectrophoresis (DEP) is widely used due to its simplicity. Here, we show the advantages in the judicious choice of the DEP microelectrode configuration in terms of limiting undesirable effects of dielectric heating on the cells, which could lead to their inactivation or death, as well as the possibility for cell clustering, which is particularly advantageous over the linear cell chain arrangement typically achieved to date with DEP. This study comprises of experimental work as well as mathematical modeling using COMSOL. In particular, we establish the parameters in a capillary-based microfluidic system giving rise to these optimum cell–cell contact configurations, together with the possibility for facilitating other cell manipulations such as spinning and rotation, thus providing useful protocols for application into microfluidic bioparticle manipulation systems for diagnostics, therapeutics or for furthering research in cellular bioelectricity and intercellular interactions.

Item Type: Article
Uncontrolled Keywords: Dielectrophoresis
Subjects: Q Science > QC Physics > QC501-766 Electricity and magnetism
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Suzilawati Abu Samah
Date Deposited: 20 Apr 2021 13:52
Last Modified: 20 Apr 2021 13:52
URII: http://shdl.mmu.edu.my/id/eprint/7627


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