Citation

Ong, Chia Sui and Ngeow, Yun Fong and Yap, Sook Fan and Tay, S. T. (2008) Molecular Identification of Nontuberculous Mycobacteria from Clinical Sources by hsp65 PRA and Sequence Analysis. International Journal of Infectious Diseases, 12 (1). e322-e323. ISSN 1201-9712

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Abstract

Identification of nontuberculous mycobacteria (NTM) species is becoming increasingly important as differences in antimicrobial susceptibility of these microorganisms may affect treatment options. In this study, PCR-RFLP analysis (PRA) of the gene encoding 65-kD heat shock protein (hsp65) was used for identification of 70 clinical NTM isolates obtained from two mycobacteriology laboratories in Malaysia. Approximately one third of the isolates were identified as M. fortuitum. A total of 24.3% and 10% of the isolates were identified as M. abscessus and M. avium complex respectively. Other isolates identified were M. kansasii, M. marinum, M. simiae, M. parascrofulaceum, M. szulgai, and M. gordonae. Six clinical isolates were untypable by this approach. Sequence analysis of the 439-bp region of the hsp65 gene and a 564-bp region of the 16S rRNA gene revealed high degrees (97%–100%) of sequence similarity of these isolates with “M. insubricum”, M. terrae, M. senegalense/M. conceptionense and M. houstonense in the public sequence databases. This study reported the identification of mycobacteria species which are considered rare or new to this region. As NTM are found widely in the environment, the clinical significance of the isolates in this study is yet to be determined.

Item Type:	Article
Additional Information:	13th International Congress on Infectious Diseases, Abstracts
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Engineering and Technology (FET)
Depositing User:	Ms Suzilawati Abu Samah
Date Deposited:	10 Aug 2011 07:44
Last Modified:	10 Jan 2017 02:48
URII:	http://shdl.mmu.edu.my/id/eprint/1998

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