Citation

Ng, Kim Tien and Ng, Liang Jie and Oong, Xiang Yong and Chook, Jack Bee and Chan, Kok Gan and Takebe, Yutaka and Kamarulzaman, Adeeba and Tee, Kok Keng (2022) Application of a VP4/VP2-inferred transmission clusters in estimating the impact of interventions on rhinovirus transmission. Virology Journal, 19 (1). pp. 1-10. ISSN 1743-422X

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Abstract

Despite the clinical burden attributable to rhinovirus (RV) infections, the RV transmission dynamics and the impact of interventions on viral transmission remain elusive. Methods A total of 3,935 nasopharyngeal specimens were examined, from which the VP4/VP2 gene was sequenced and genotyped. RV transmission clusters were reconstructed using the genetic threshold of 0.005 substitutions/site, estimated from the global VP4/VP2 sequences. A transmission cluster is characterized by the presence of at least two individuals (represent by nodes), whose viral sequences are genetically linked (represent by undirected edges) at the estimated genetic distance threshold supported by bootstrap value of ≥ 90%. To assess the impact of facemask, pleconaril and social distancing on RV transmission clusters, trials were simulated for interventions with varying efficacy and were evaluated based on the reduction in the number of infected patients (nodes) and the reduction in the number of nodes-connecting edges. The putative impact of intervention strategies on RV transmission clusters was evaluated through 10,000 simulations.

Item Type:	Article
Uncontrolled Keywords:	Acute respiratory tract infection, Rhinovirus, VP4/VP2 gene, Transmission clusters, Interventions
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Information Science and Technology (FIST)
Depositing User:	Ms Nurul Iqtiani Ahmad
Date Deposited:	06 Apr 2022 00:25
Last Modified:	06 Apr 2022 00:25
URII:	http://shdl.mmu.edu.my/id/eprint/10038

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