Measurement of protein in latex glove using computerized colorimetric protein estimation method


Toa, C. K. and Chan, Yee Kit and Sim, Kok Swee and Mok, K. L. (2020) Measurement of protein in latex glove using computerized colorimetric protein estimation method. Engineering Letters. pp. 1-10. ISSN 2193-567X

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Proteins of the latex glove will become a potential allergy threat to people who are frequently exposed to latex. A robust way to control protein content is by estimating the amount of protein. Several established protein estimation methods such as modified Lowry method and smartphone for point-of-care quantification of protein method have been proposed. However, most of the conventional methods involve many chemical processes, and time-consuming. In order to overcome those limitations, a novel method named computerized colorimetric protein estimation (CCPE) is designed. The glove sample goes through chemical test to perform protein detection. Later, the sample is converted into digital image through scanning process by using flatbed scanner. A new algorithm named smoothing colorimetric adaption (SCA) has been proposed to perform image filtering on smoothing the wrinkled surface of glove and color difference formula to calculate the delta E, ∆E data of glove sample. The experimented data is then plotted in a standard curve and validated with the protein concentration data from the Malaysia Rubber Board (MRB). Based on the results, a strong correlation with R2 percentage equal to 97% is established between both data. This indicates that the CCPE method provides reasonably practicable estimation of the protein concentration.

Item Type: Article
Uncontrolled Keywords: Colorimetric analysis, Computerized colorimetric, Color difference, Latex glove, Protein concentration
Subjects: Q Science > QD Chemistry > QD71-142 Analytical chemistry
Divisions: Faculty of Engineering and Technology (FET)
Depositing User: Ms Rosnani Abd Wahab
Date Deposited: 14 Dec 2020 10:16
Last Modified: 14 Dec 2020 10:16


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